Mouse HPX ELISA kit

Detail Description about this product

INTENDED USE

For the quantitative determination of Mouse Hemopexin,HPX concentrations in serum, plasma, cell culture supernates.

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HPX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hemopexin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hemopexin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HPX bound in the initial step. The color development is stopped and the intensity of the color is measured.

For research use only. Not for diagnostic use.

MANUAL

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PRECAUTIONS

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

MORE INFORMATION

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