Human PG-E2 ELISA Kit

Detail Description about this product

INTENDED USE

For the quantitative determination of Human Prostaglandin E2 (PG-E2) in serum, plasma, cell culture supernates.

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for (PG-E2) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any (PG-E2) present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for (PG-E2) is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of (PG-E2) bound in the initial step. The color development is stopped and the intensity of the color is measured.

For research use only. Not for diagnostic use.

MANUAL

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PRECAUTIONS

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

MORE INFORMATION

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